IgE, Antibody

Product Information
IgE, Antibody.  

Description: IgE, Antibody.  

MoAb Isotype: IgG for MAbs BE5, 4G7 and 4H10 IgG1 for MAb XTE4 IgG2a for MAbs 4F4, 5D4 and E411  

Clones Offered: 4F4, 5D4, E411, XTE4, BE5, 4G7, 4H10 Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with IgE from myelomatous human serum.  

Specificity: MAb E411 class specific for human IgE, Fc-region specific MAb 4F4 - IgE Cε3-domain specific MAb 5D4 - IgE Cε2-domain specific MAb XTE4 - epsilon chain specific Specific antibody titer is 1/1 000 000 by indirect ELISA.  

Applications: Detection of total IgE level and IgE antibodies in ELISA. Recommended pairs (coating - conjugate): 5D4 – 4F4, 4G7– BE5. MAb E411 can be used for coating. MAb 4F4 can be used for IgE purification by affinity chromatography. MAb XTE4 can be used in Western blotting.  

Purity: MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose MAb XTE4 by ion exchange chromatography  

Presentation: MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 in PBS, pH 7.4, 0.1 % sodium azide (NaN3) XTE4 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % sodium azide (NaN3)  

Shipping Conditions: + 4 °C  

Comments: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.05-0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Allergol. et Immunopathol. 1978, vol 1, pp. 423-426 Allergy and Clin. Immunol. News, 1992, vol.4, 21-25

Price: $242.00

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