Human recombinant proBNP (human brain natriuretic peptide precursor)

Recombinant E.coli.

Calibrator or standard in immunoassays, immunogen for antibody production.

Purity > 95 % (Tricine-SDS-PAGE).
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of proBNP molecule.
Predicted molecular mass 12036 Da confirmed by MALDI-MS.
Recombinant human proBNP contains additional methionine residue at the N-terminus (in comparison with native proBNP).
Human recombinant proBNP also contains one intramolecular disulfide bond between residues Cys87 and Cys103.

Immunoaffinity purification followed by ion-exchange chromatography

10 mM potassium phosphate, pH 7.4, 150 mM NaCl.

-70 °C (between -65 and -80 °C allowed)

Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
To prepare diluted samples of proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) Assay Notes.
Antibodies for New Type of BNP immunoassay – Single Epitope Sandwich Assay Notes.

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