Description: SmarTaq DNA Polymerase is complex mixture of a thermostable 94 kDa BioTaq DNA Polymerase purified from E. coli PVG-AI recombinant strain expressing Thermus aquaticus polymerase gene and specific monoclonal antibodies.
SmarTaq from Advanced ImmunoChemical, Inc. is inactive under conditions of amplification reaction preparation. It provides improved specificity when compared to standard DNA polymerases. SmarTaq can eliminate amplification artifacts such as primer-dimer formation and mis-priming.
An advantage of SmarTaq is the absence of an additional heating step for polymerase activation. Heat activation of the enzyme occurs during the first denaturation step. An active complex of SmarTaq dissociates automatically over +70 °C, allowing activation of DNA polymerase.
Storage and dilution buffer: 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Nonidet P-40, 0.5 % Tween-20.
Unit definition: One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 74 °C.
Amplification buffer 10x: NH4-buffer: 166 mM (NH4)₂SO4, 670 mM Tris-HCl (pH 8.8 at 25 °C), 0.1 % Tween-20.
Applications: Highly specific PCR, multiplex PCR (highly recommended), high sensitivity applications.
Quality control: Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
Storage:-20 °C (-15 … -30 °C allowed)
Material safety note: This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
- Cat. #10-STP1. SmarTaq DNA Polymerase
- (Product can also be ordered at the Fisher Scientific brand website, cat. #501539821.)
- (Product can also be ordered at the Fisher Scientific brand website, cat. #501539822.)