GAPDH Western Blot Set

Catalog Number: 2-RGM2-set


General Category:

    Antigens » Research and Development

Disease Category:

    Antigens » Research and Development
GAPDH immunodetection in Western blotting (as loading control and other applications) and DOT blot, using GAPDH sample as internal standard or calibrator.

Please see “GAPDH Western Blot Set Assay Notes” for detailed protocol of 5-minute GAPDH immunodetection method.


  1. Detection MAb 6C5 conjugated with horseradish peroxidase (HRP).
  2. Protein standard human GAPDH.

Sample Preparation:

HRP-conjugated MAb 6C5 is ready to use in Western blotting.

Recommended working dilution is 1:500 for DAB staining and 1:5000 for ECL.


GAPDH from different species.

1 x 1 mg vial, in 50% PBS, pH 7.4, 50% glycerol.

Antigen Standard is ready to use after reconstitution in distilled water.

The vial contains 5 µg of human GAPDH. Add 50 µl of water per vial and dissolve the pellet with gentle stirring. After dissolving centrifuge the vial briefly to collect all the liquid to the bottom of the vial.

1 x 5 µg vial, lyophilized from protein solution in 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% sucrose, 1% 2-mercaptoethanol, 0.1% bromophenol blue

Storage Conditions:

-20°C (-15 … -30°C allowed)

Material safety note:
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.

Warranty and Limitation of Liability:

Advanced ImmunoChemical, Inc. makes no warranty of any kind, expressed or implied, including warry of merchantability or fitness for any particular purpose. Data provide with the product is only guideline on how the product may perform in your system. Since every diagnostic system depends on many reagents, it is impossible to predict with any accuracy how our product will react in your system.

Click below to download a PDF product data sheet
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