Catalog Number: 8-NTpB-rh
Source:Recombinant E.coli.Applications:Calibrator or standard in immunoassays, immunogen for antibody production.Analysis:Purity > 95 % (Tricine-SDS-PAGE).
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.
Predicted molecular mass 8589 Da confirmed by MALDI-MS.
Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).Purification:Immunoaffinity purification followed by ion-exchange chromatography.Presentation:10 mM potassium phosphate, pH 7.4, 150 mM NaClStorage Conditions:-70 °C (between -65 and -80 °C allowed)Other information:Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).Material safety note:This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.Additional product information:
Antigens » Cardiac Markers » ProBNP, BNP and NT-proBNP
Cardiac Markers » Antigens » ProBNP, BNP and NT-proBNP
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