Monoclonal mouse anti-cardiac Troponin T (cTnT), in vitro

300cc, 329cc, 406cc, 1C11cc, 1F11cc
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic human TnT peptide a.a.r. 119-138 conjugated with carrier protein (for MAbs 300cc and 329cc), or synthetic human TnT peptide a.a.r. 106-183 conjugated with carrier protein (for MAb 406cc), or free human cTnT (MAbs 1C11cc and 1F11cc).

MAbs 406cc, 1C11cc and 1F11cc have no cross-reaction with skeletal TnT. Cross-reactivity of MAbs 300cc and 329cc with skeletal TnT is less than 1%.

IgG1 for MAb 300cc, 329cc, 1C11cc

IgG2a for MAb 406cc

IgG2b for MAb 1F11cc

TnT immunoassay. All MAbs recognize TnT in sandwich immunoassay.

Recommended pairs for sandwich immunoassay (capture – detection):
406cc – 300cc
329cc – 406cc

MAbs 1C11cc and 1F11cc are working in Western blotting.

Chromatography on protein A Sepharose.

PBS, pH 7.4, 0.09 % sodium azide (NaN3)

+4 °C (+2 … +8 °C allowed)

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.