Monoclonal mouse anti-D-dimer

Catalog Number: 2-DD1

From: $236.00

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General Category:

    Monoclonal antibodies » Blood Coagulation and Anemia
    Monoclonal antibodies » Cardiac Markers » D-dimer

Disease Category:

    Cardiac Markers » Monoclonal antibodies » D-dimer
    Blood Coagulation and Anemia » Monoclonal Antibodies
MAbs:
DD1, DD2, DD3, DD4, DD5, DD6, DD22, DD41, DD44, DD46, DD93
New MAbs: DD189. DD255
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells
of Balb/c mice immunized with homogenized fibrin clot, D-dimer or high molecular weight fibrin degradation products.
Specificity:
All MAbs recognize D-dimer and high molecular weight fibrin degradation products.
MAb DD93 recognizes a cross-linked region of D-dimer.
MAbs DD1, DD2, DD3, DD22, DD41, DD44, DD46, DD93, DD189 and DD255 do not cross-react with fibrinogen.
MAbs DD4, DD5 and DD6 show cross-reaction with fibrinogen.
MAb Isotypes:
IgG1 for MAbs DD93, DD189, DD255
IgG2a for MAbs DD1, DD6, DD22, DD41, DD46
IgG2b for MAbs DD2, DD3, DD4, DD5, DD44
Applications:
Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin degradation products.
All antibodies recognize D-dimer in ELISA. All MAbs recognize D-dimer in Western blotting under non-reducing conditions. MAbs DD22, DD41, DD44, DD46 and DD189 interact with beta-chain of D- dimer in Western blotting under reducing conditions. MAbs DD93 and DD255 interact with gammachain of D-dimer in Western blotting under reducing conditions.
Recommended pairs to be used in a sandwich immunoassay for D-dimer detection in humanplasma (capture-detection):
One-step sandwich immunoassays:
DD189 – DD255 (Equal specificity to D-dimer and high MW fibrin degradation products). DD2 –DD41 (Slightly more specific to high MW fibrin degradation products at high concentrations).
Two-step sandwich immunoassays*:
DD2 – DD4 (Approximately equal specificity to D-dimer and high MW fibrin degradation products).
*To be analysed in a two-step sandwich immunoassay, plasma must be diluted 1:1 or more with 10 mM Tris-HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0.5 M or more.)
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage Conditions:
+ 4 °C

Material Safety Note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

References:
Search D-dimer antibody references from PubMed.
Click below to download a PDF product data sheet
   
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