Monoclonal mouse anti-human adiponectin

Adn23, Adn27, Adn36, Adn63, Adn94, Adn214, Adn222, Adn279, Adn305
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human recombinant adiponectin from E. coli (MAbs Adn23) or with native human adiponectin (MAbs Adn27, Adn36, Adn63, Adn94, Adn214, Adn222, Adn279 and Adn305).

Human serum adiponectin

IgG1 for MAbs Adn63, Adn94, Adn214, Adn222, Adn279, Adn305
IgG2a for MAbs, Adn23, Adn27, Adn36

MAbs Adn23, Adn63, Adn214 and Adn222 react with human serum adiponectin in Western blotting.
MAbs Adn23 and Adn63 recognise the whole spectrum of oligomeric forms of adiponectin after nonheating, non-reducing SDS-PAGE.
Recommended pairs for total adiponectin immunodetection in sandwich immunoassay
(capturedetection):
Adn36 – Adn27
Adn63 – Adn94
Adn94 – Adn63
Adn305 – Adn279
Recommended pair for high/medium molecular weight adiponectin immunodetection in sandwich immunoassay (capture – detection):
Adn23
Recommended pairs for low molecular weight adiponectin immunodetection in sandwich immunoassay (capture – detection):
Adn214 – Adn27 Adn222 – Adn36

Chromatography on protein A Sepharose

Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3)

+ 4 °C

This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Adiponection Assay Notes.  Retinol Binding Protein (RBP4) Assay Notes.

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