Catalog Number: 2-NT-proBNP
MAbs:15F11, 13G12, 15D7, 15C4, 24E11, 28F8, 29D12, 18H5, 16F3
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (MAbs, 29D12), a.a.r. 13-27 (MAbs 15F11, 13G12,18H5, 16F3), a.a.r. 28-45 (MAb 15D7) and a.a.r. 61-76 (MAbs 24E11, 28F8, 15C4). The peptides used for immunization were conjugated with carrier protein.Specificity:Human NT-proBNP and proBNP.MAb Isotypes:IgG1 for MAbs 15D7, 18H5, 16F3
IgG2a for MAbs 29D12, 13G12, 24E11, 28F8
IgG2b for MAbs 15F11, 15C4Applications:NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.
Best pairs for sandwich immunoassay (capture-detection):
15F11 – 24E11, 15C4 – 29D12, 15C4 – 13G12, 15C4 – 18H5.
These pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.
MAbs specific to peptides 5-12 (MAb 29D12) and 13-27 (MAbs 15F11, 13G12, 18H5, 16F3) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 2-BNP). The best pairs (capture-detection):
50E1 (Cat.# 2-BNP) – 16F3 (Cat.# 2-NT-proBNP)
50E1 (Cat.# 2-BNP) – 18H5 (Cat.# 2-NT-proBNP)
2G9 (Cat. # 2-BNP)Purification:Chromatography on protein A SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °CMaterial Safety Note:This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.Additional product information:Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) Assay Notes. Antibodies for New Type of BNP immunoassay Single Epitope Sandwich Assay Notes.
Monoclonal antibodies » Cardiac Markers » ProBNP, BNP and NT-proBNP
Cardiac Markers » Monoclonal antibodies » ProBNP, BNP and NT-proBNP
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