Monoclonal mouse anti-human pregnancy-associated plasma protein A (PAPP-A)

Catalog Number: 2-PAPP

From: $245.00

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General Category:

    Monoclonal antibodies » Cardiac Markers » PAPP-A

Disease Category:

    Fertility and Pregnancy
MAbs:

10E1cc, 10E2cc, 5H9, 4G11, 3C8, 10H9, 11E4, 7A6
New MAb: PAPP52
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex or purified human atherosclerotic tissue form of PAPP-A (MAb PAPP52).
Specificity:
Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and proMBP subunits). MAbs 11E4 and 5H9 are specific to proMBP subunit.
MAb Isotypes:

IgG1 for MAb PAPP52
IgG2a for MAbs 4G11, 3C8, 10H9, and 7A6
IgG2b for MAbs 5H9, 11E4, 10E1cc and 10E2cc
Applications:
Recommended pairs for detection of human htPAPP-A antigen (capture – detection):
10E2cc – 5H9
5H9 – 10E2cc
5H9 – 7A6
10E1cc – 11E4
Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A) (capture –detection):
10E2cc – 10E1cc
4G11 – 3C8
4G11 – 10H9
10E1cc – 7A6
PAPP-A immunodetection in Western blotting. MAb 18A10 recognizes PAPP-A subunit in reducing and non-reducing conditions after antigen transfer onto nitrocellulose membrane after SDS-gel electrophoresis. MAbs 4G11, and 10E1 recognize htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage Conditions:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
References:
Search PAPP-A references from PubMed.
See all PAPPA-A References.
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