Monoclonal mouse anti-neutrophilin gelatinase-assocated lipocalin (NGAL)

N417, N422, N432, N457, N461, N308, N316

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human NGAL expressed in mammalian cell line (MAbs N417, N422, N432, N457, N461).

MAbs N308 and N316 are recombinant rabbit full-sized MAbs expressed in mammalian cells. Rabbits were immunized with recombinant human NGAL expressed in mammalian cell line.

MAbs N308, N432 and N457: human NGAL monomer, human NGAL homodimer.

MAbs N316, N417, N422, N461: Human NGAL monomer, human NGAL homodimer, human NGAL/MMP-9 heterodimer

IgG1 for MAbs N417, N422, N432, N457, N461
IgG for MAbs N308, N316

Recommended pairs in sandwich immunoassays (capture-detection):
N308 – N432 (monomer and homodimer)
N316 – N417 (monomer, homodimer and heterodimer)
N316 – N457 (homodimer)
N316 – N461 (monomer, homodimer and heterodimer)
N422 – N417 (monomer, homodimer and heterodimer)
N422 – N461 (monomer, homodimer and heterodimer)
MAbs N308, N316 and N417 are working in Western blotting.

Chromatography on protein A Sepharose

PBS, pH 7.4, 0.09 % sodium azide (NaN3)

+4 °C (+2 … +8 °C allowed)

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material. Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.