Monoclonal mouse anti-Newcastle disease virus (NDV)

9F7, 1C10, 2H4, 6H12
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Newcastle disease virus (La-sota strain).

MAbs 9F7, 1C10, 2H4 and 8H2 are specific to viral glycoprotein haemagglutinin-neuraminidase.
MAb 6H12 is specific to RNP.

IgG1 for MAb 9F7
IgG2a for MAbs 1C10, 8H2, 6H12
IgM for MAb 2H4

MAbs 9F7  and 1C10 are working in Western blotting.
MAbs 9F7, 1C10 and 2H4 are working in haemagglutinin inhibition test.
All MAbs perform in ELISA.
MAbs 2H4 and 8H2 are recommended as coating antibodies and MAbs 9F7 and 1C10 as conjugate antibodies in sandwich immunoassay.
MAb 6H12 performs in Immunofluorescence (IF) and in Peroxidase –Immunohistochemistry staining of infected cells.

Protein G chromatography

PBS, pH 7.4 with 0.09 % sodium azide (NaN3)

+ 4 °C (+2°  – +4° allowable)

This product is sold as an antibody preparation for research and further manufacturing purposes only. Standard Laboratory Practices must be followed when handling this material.
Although the amount of  sodium azide is low, appropriate care must be taken when handling this product.