Catalog Number: 2-SAA-a
MAbs:VSA34, SAA19, SAA21, SAA11, VSA31, VSA38, VSA2, VSA43
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with either human SAA (SAA19, SAA21, SAA11), or canine SAA (VSA34, VSA31, VSA38), or synthetic peptides derived from the region 79-104 a.a.r. of canine SAA (VSA2, VSA43).Specificity:Canine SAA (all MAbs)
Feline SAA (VSA34, SAA19, VSA31, VSA38, SAA21, SAA11)
Equine SAA (all MAbs)MAb Isotypes:IgG1 for MAb VSA2
IgG2a for MAbs SAA19, VSA31, VSA38
IgG2b for MAbs VSA34, SAA21, VSA43, SAA11Applications:Recommended pairs for sandwich immunoassay (capture – detection):
SAA19 – VSA34: canine, feline, equine
SAA21 – VSA34: canine, feline, equine
VSA2 – VSA38: canine, equine
VSA2 – VSA31: canine, equine
VSA38 – VSA43: canine, equine
All MAbs react with SAA in Western blotting.Purification:Chromatography on protein G SepharosePresentation:PBS, pH 7.4, 0.1 % sodium azide (NaN3)Storage Conditions:+ 4 °COther information:We recommend to avoid using bovine serum albumin (BSA) as a buffer component or blocking agent for SAA immunoassay. In buffers, BSA can be replaced with 1% casein.
Immunoassay plates blocking with casein is needed to prevent non-specific binding of SAA to the wells of an immunoassay plate.
For pairs SAA19 – VSA34 and SAA21 – VSA34, we recommend to start assay optimization using 0.01% CHAPS in antigen dilution and washing buffers. According to our data, Tween 20 lowered the signal in the wells when it was used in concentration 0.05-0.1% in antigen dilution buffer. When lower concentration of Tween 20 was used (0.005-0.025%), non-specific binding of SAA to the wells of an immunoassay plate was observed.
For pairs VSA2 – VSA38, VSA2 – VSA31 and VSA38 – VSA43, we recommend to use antigen dilution buffer containing 1% casein and 0.05-0.1% Tween 20.Material Safety Note:This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Monoclonal antibodies » Veterinary » Canine
Monoclonal antibodies » Veterinary » Equine
Monoclonal antibodies » Veterinary » Other animal specific antibodies
Veterinary » Monoclonal antibodies » Canine
Veterinary » Monoclonal antibodies » Other animal specific antibodies
Veterinary » Antigens » Equine
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