Neuron-specific enolase (NSE)

Catalog Number: 8-NSE

From: $649.00

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General Category:

    Antigens » Neuroscience
    Antigens » Tumor Markers

Disease Category:

    Neuroscience » Antigens
    Tumor Markers » Antigens
Source:
Post mortal human brain.
Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.
Analysis:
Purity > 95 % (SDS-PAGE).
Activity:
Specific activity is determined for each lot at 25 °C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-Dglycerate per min at 25 °C (J.Biochem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.
Immunological activity:
Tested in ELISA and immunoblotting with anti-NSE antibodies.
Purification:
Ion-exchange chromatography and gel-filtration.
Presentation:
Presentation: Solution in 0.1 M Tris-HCl, 5 mM Mg2SO4, 0.25 M KCl, pH 5.1 adjusted with sodium phosphate.
Storage Conditions:
-20 °C (between -15 and -30 °C allowed)
Other information:
Avoid repeated freezing and thawing.
Material safety note:
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
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