Recombinant human cardiac troponin T (cTnT)

Catalog Number: 8-TTC-rh

$443.00

General Category:

    Antigens » Cardiac Markers » Troponin T

Disease Category:

    Cardiac Markers » Antigens » Troponin T
Synonym names:
Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3
Source:
Expressed in E. coli
Protein sequence:
Product of gene TNNT2; 288 a.a.r.
MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN ММHFGGYIQK QAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK
Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).
Applications:
Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard
Analysis:
Purity > 95 % (SDS-PAGE).
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) – 0.490 (measured at 280 nm)
Immunological activity:
Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform
Purification:
Immunoaffinity purification followed by ion-exchange chromatography
Presentation:
Lyophilized from 0.01 M HCl
Reconstitution:
Reconstitute with Tris/urea buffer (20 mM Tris pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol.
Storage Conditions:
Lyophilized -20 °C (between -15 and -30 °C allowed)
Reconstituted -70 °C (between -65 and -80 °C allowed)
Other information:
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
Material Safety Note:
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
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