Recombinant human interleukin 6 (IL-6)

Expressed in a mammalian cell line

Synthetic DNA fragment encoding whole human interleukin-6 (Accession number UniProtKB P05231, IL6_HUMAN) with 6His-tag on C-terminus was used for protein expression.
Contains C-terminal His6 tag
Theoretical molecular weight 21 950 Da
Theoretical pI 6.77

Calibrator in immunoassay

Purity >90%
Concentration is determined spectrophotometrically at A280.

Metal affinity chromatography

PBS, pH 7.4, 0.1% Tween 80

-70°C (-65 … -80 °C allowed)

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing