Catalog Number: 8-S100ab-h
Source:Human brain tissue.
Blood samples from tissue donors were tested and found to be negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.Applications:S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21kDa. In human brain tissue S-100 protein is mainly presented by two isoforms – ββ homodimer (S-100b) and αβ heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 rotein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, in diagnosis and prognosis of clinical outcome in acute stroke.
S-100 protein is suitable for use as a standard in immunoassay, as an immunogen for antiserum production and tracer for iodination.Analysis:Purity > 95 %. After native gel electrohoresis by Ornstein-Davis S-100 protein is presented by two bands corresponding to αβ- and ββ-forms.
S-100 protein concentration was determined by Lowry method using BSA as a standard.Presentation:Lyophilized from 5 mM Tris-HCl, pH 7.5 with 5 mM 2-mercaptoethanol and 5 mM EDTA.
Product is recommended to reconstitute with 5 mM 2-mercaptoethanol.Storage Conditions:-20 °C (between -15 and -30 °C allowed)Material safety note:This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Antigens » Neuroscience
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