T aquaticus DNA Polymerase, recombinant

Catalog Number: 10-TaP1

From: $425.00

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General Category:

    Molecular Biology

Disease Category:

    Molecular Biology
Description:
Taq DNA Polymerase (recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus strain YT-1) consists of a single polypeptide chain with a molecular weight 94 kDa. It is a highly processive 5′-3′ DNA polymerase lacking 3′-5′ exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- and exonucleases. This Taq DNA polymerase has a nontemplate-dependent activity, which adds a single deoxyadenosine (A) to the 3′ ends of PCR products (3′-A overhangs). This property allows easily and efficiently to ligate the PCR products in TA cloning vectors. Amplification of target DNA fragments from 100 bp to 4 Kb can be achieved with this enzyme.

 

Storage and dilution buffer:
20 mM HEPES, pH 7.9, 125 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5 % Tween 20, 50 % glycerol.
Unit Definition:
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 72 °C.
Reaction buffer 10x (Mg2+ minus):

670 mM Tris-HCl, pH 8.8, 166 mM (NH4)2SO4, 0.1 % Tween 20.

Other common PCR buffers for Taq-DNA polymerases can be used as well.

Optimal concentration of MgCl2 is 1.0-2.5 mM.

Applications:
PCR and DNA labelling.
Quality Control:
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
Concentration:
5 units/µl
Storage Conditions:
-20 °C (-15 … -30 °C allowed)
Material safety note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

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