Recent Citations

A modified non-human warm-blooded vertebrate animal in which a biologically active
human APP polypeptide is expressed, and in which function of its inducible Nitric Oxide
Synthase (iNOS) protein is reduced as compared to a non-modified animal, methods of …

Embodiments of the invention provide methods, polyclonal antibodies, monoclonal
antibodies, assays, and kits for detecting HPV infection, including infection by various HPV
genotypes, early and/or late HPV-associated or HPV-specific proteins or antibodies. …

Bispecific antibodies (BsAbs) have proven highly efficient T cell recruiters for cancer
immunotherapy by virtue of one tumor antigen-reactive single chain variable fragment (scFv)
and another that binds CD3. In order to enhance the antitumor potency of these tandem …

Abstract Mitochondrial dysfunction is implicated in multiple neurodegenerative diseases. In
order to maintain a healthy population of functional mitochondria in cells, defective
mitochondria must be properly eliminated by lysosomal machinery in a process referred to …

Background. We previously reported that exercise training (ET) facilitates the clearance of
damaged proteins in heart failure. Here, we characterized the impact of ET on cardiac
protein quality control during compensated ventricular hypertrophy in spontaneously …

Abstract The let-7 microRNA (miRNA) family has been implicated in the regulation of diverse
cellular processes and disease pathogenesis. In cancer, loss-of-function of let-7 miRNAs
has been linked to tumorigenesis via increased expression of target oncogenes. …

Abstract Success in developing therapeutic approaches to target brain tumor-associated
inflammation in patients has been limited. Given that the inflammatory microenvironment is a
hallmark signature of solid tumor development, anti-inflammatory targeting strategies have …

Abstract Elevated levels of chemokine receptor CCR9 expression in solid tumors may
contribute to poor patient prognosis. In this study, we characterized a novel CCR9-mediated
pathway that promotes pancreatic cancer cell invasion and drug resistance, indicating that …

Abstract Fibrosis of the aging heart impedes cardiac function and increases the risk of
arrhythmias and heart disease. Previously, we demonstrated that exercise-induced
reduction of collagen I in the aging heart was linked to a suppression of oxidative stress …

Abstract Myosin phosphatase (MP) is a key target of signaling pathways that regulate
smooth muscle tone and blood flow. Alternative splicing of MP targeting subunit (MYPT1)
exon 24 (E24) generates isoforms with variable presence of a C-terminal leucine zipper ( …

Pseudomonas aeruginosa toxin ExoY, a cUMP/cGMP-producing nucleotidyl cyclase.
C Woelfel, DW Frank, S Wolter, V Kaever, R Seifert NAUNYN-SCHMIEDEBERGS
ARCHIVES OF PHARMACOLOGY 381, 29-29, SPRINGER, 3/2010.

Rationale: Pseudomonas aeruginosa is an opportunistic pathogen that causes acute and
chronic pulmonary infections. During pulmonary infection, Pseudomonas uses a type III
secretion system (T3SS) to transfer an adenylyl cyclase termed ExoY to the cytoplasm of …

Rationale: ExoY is a Pseudomonas aeruginosa toxin that is introduced into host cells
through the type 3 secretion system (T3SS). Once inside the host cell cytoplasm ExoY
generates cyclic nucleotides that cause tau phosphorylation and microtubule breakdown. …

The effector protein ExoY secreted by Pseudomonas aeruginosa augments the
inflammatory reaction in the respiratory tract of mice. J Rothschuh, A Munder, B
Tuemmler, D Frank, R Seifert, C Hartwig NAUNYN-SCHMIEDEBERGS …

Abstract Our laboratory recently demonstrated that Pseudomonas aeruginosa exoenzyme Y
(ExoY) stimulates Tau hyperphosphorylation and insolubility-hallmarks of a “Tauopathy”.
Unlike neurodegenerative Tauopathies, we did not observe formation of Tau aggregates …

Milwaukee, WI causes bronchopneumonia that can progress to acute lung injury. interacts
with and disrupts the Pseudomonas aeruginosa P. aeruginosa lung capillary endothelial cell
barrier resulting in alveolar edema and hemorrhage. uses a type III secretion system ( …

Abstract ExoY from Pseudomonas aeruginosa is injected into host cells via the type III
secretion system (Yahr TL et al., Proc Natl Acad Sci USA 95: 13899 (1998)). ExoY is
structurally similar to CyaA from Bordetella pertussis and edema factor (EF) from Bacillus …

MATERIALS AND METHODS Bacterial strains, plasmids, and growth conditions. All
bacterial strains and plasmids used in this study are listed in Table 1. F. tularensis LVS was
routinely grown at 37 C in modified Mueller-Hinton (MH) broth or on agar (Difco …

Abstract Here, we tested the hypothesis that Pseudomonas aeruginosa type 3 secretion
system effectors ExoY and ExoU induce endothelial tau oligomerization and release,
causing transmissible cytotoxicity characteristic of an infectious tauopathy. Both the …

Copyright:© 2015 Anderson et al. This is an open access article distributed under the terms
of the Creative Commons Attribution License, which permits unrestricted use, distribution,
and reproduction in any medium, provided the original author and source are credited

RATIONALE: Strongly adherent pulmonary microvascular endothelial cells (PMVECs)
interact to form the pulmonary capillary barrier, necessary to maintain efficient gas exchange
in the lung. Transmembrane adenylyl cyclase activity generates cAMP restricted to the …

ABSTRACT Pathogen life cycles in mammalian hosts have been studied extensively, but
studies with arthropod vectors represent considerable challenges. In part this is due to the
difficulty of delivering a reproducible dose of bacteria to follow arthropod-associated …

Abstract The MiniChromosome Maintenance 2-7 (MCM2-7) complex provides essential
replicative helicase function. Insufficient MCMs impair the cell cycle and cause genomic
instability (GIN), leading to cancer and developmental defects in mice. Remarkably, …

Abstract Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is a DNA repair enzyme that processes
blocked 3? ends of DNA breaks. Functional loss of Tdp1 causes spinocerebellar ataxia with
axonal neuropathy type 1 (SCAN1). Based on the prominent cytoplasmic expression of …

Abstract Alexander disease (AxD) is a rare neurodegenerative disorder characterized
pathologically by the presence of eosinophilic inclusions known as Rosenthal fibers (RFs)
within astrocytes, and is caused by dominant mutations in the coding region of the gene …

Abstract Anti-HER2 monoclonal antibodies (mAb) have been shown to reduce tumor size
and increase survival in patients with breast cancer, but they are ineffective against brain
metastases due to poor brain penetration. In previous studies, we identified a peptide, …

Abstract Aversive stressful experiences are typically associated with increased anxiety and a
predisposition to develop mood disorders. Negative stress also suppresses adult
neurogenesis and restricts dendritic architecture in the hippocampus, a brain region …

ABSTRACT Phospholipase A 2 enzymes are ubiquitously distributed throughout the
prokaryotic and eukaryotic kingdoms and are utilized in a wide array of cellular processes
and physiological and immunological responses. Several patatin-like phospholipase …

Abstract Mammalian cells contain the cyclic pyrimidine nucleotides cCMP and cUMP. It is
unknown whether these tentative new second messenger molecules occur in vivo. We used
high performance liquid chromatography quadrupole tandem mass spectrometry to …