SmarTaq Polymerase AssayNotes

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(Deoxynucleoside-triphosphate: DNA Deoxynucleosidyltransferase E.C.

SmarTaq is a Taq polymerase modi ed by blocking the specific polymerase activity with highly specific monolonal antibodies against Taq polymerase active site. SmarTaq remains inactive during set-up of PCR reaction at ambient temperatures (20-25 °C). An activation of enzyme occurs during the first PCR cycle by dissociation of enzyme-antibody complex when the temperature rises above 70 °C. SmarTaq significantly improves PCR results by preventing primer-dimer artifact formation and non-specific amplification. SmarTaq also gives better yields and greater specificity when amplifying difficult templates.

SmarTaq can be stored for a long period (more than 3 months) at + 4 °C. In contrast to the chemically modi ed Taq polymerase, SmarTaq is compatible with most of the buffers traditionally applied for amplification.

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