A1-PINP, human, recombinant

Expressed in a mammalian cell line.

Synthetic DNA fragment corresponding to region 1-161 of human Collagen alpha-1(I) chain including
signal peptide was used according to UniProtKB P02452 accession number. Contains C-terminal
Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-His 6 -tag.
Theoretical MW: 15839 Da including C-terminal tag
Theoretical pI: 4.65.

Calibrator or standard in immunoassays.

• Purity >92%
  Concentration is determined spectrophotometrically at A280. Abs 1 mg/ml = 0.47
  Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to recombinant human
  A1-PINP.

Metal affinity chromatography

Lyophilized from PBS, pH 7.4.
It is recommended to reconstitute this product with deionized water to its initial concentration.

Lyophilized and reconstituted at -70 °C (-65 … -80 °C allowed)

Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

This product is sold for research or further manufacturing use only. Standard Laboratory Practices should be followed when handling this material.