Catalog Number: 8-CNT
Source:Recombinant protein with N-terminal proprietary affinity tag expressed n E.coli.Applications:Standard for the calibration of canine NT-proBNP immunoassatys.Analysis:
Recombinant NT-proBNP corresponds to the fragment 1-85 a.a.r. of canine proBNP and contains additional affinity tag sequence of 16 a.a.r. at the N-terminus.
Totally, recombinant NT-proBNP conists of 101 a.a.r.
Predicted molecular mass 10545 Da is confirmed by MALDI-MS.
Purity > 95% (Tricine-SDS-PAGE).
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of canine NT-proBNP molecule (Cat# 2-CNT).Presentation:
10 mM potassium phosphate, pH 7.4, 150 mM NaCl.Storage Conditions:
-70 °C (-65 … -80 °C allowed)Other information:
Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tric-HCl, pH 7.8, 0.15 M NaCl, 0.01% Tween 40, 0.5% bovine serum albumin).Material safety note:This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.Additional Product Information:
Canine NT-proBNP Assay NotesReferences:Related Products:
Cat# 2-CNT Monoclonal mouse anti-canine N-terminal proBNP (NT-proBNP)
Antigens » Veterinary
Veterinary » Antigens » Canine