Monoclonal mouse anti- cardiac troponin I (cTnI)

Catalog Number: 2-TIc

From: $254.00

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General Category:

    Monoclonal antibodies » Veterinary » Other animal specific antibodies
    Monoclonal antibodies » Cardiac Markers » Troponin I and Troponin complex

Disease Category:

    Veterinary » Monoclonal antibodies » Other animal specific antibodies
    Cardiac Markers » Monoclonal antibodies » Troponin I and Troponin complex

Alternatives in vitro!
Please note that MAbs M155cc, 19C7cc, 16A12cc, 16A11cc and MF4cc are also available as in vitro produced products under Cat.# 2-Tic-cc. For immunoassays utilizing of these MAbs, we recommend selecting the in vitro produced product.

MAbs:
8E10, C5, 4C2, 19C7, 16A11, 3C7, P4-14G5, M18, M155, M46, MF4, 16A12, P4-9F6, 228, 625, 458, 267, 596, 84, 415, 581, 10F4, 247, 560, 17F3, p45-10
New MAbs: 916, 909, 820, 810, 801
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI, native human cTn complex, or human cTnI peptides.

Specificity:
For epitope specificity see the printable version. None of the MAbs (except M46, 267, 596, and C5) cross-react with skeletal muscle troponin I. Cross-reactivity with skeletal troponin I is less than 10% for MAbs M46, 267 and 596, and more than 50% for MAbs C5.  MAb 247 reacts only with free cardiac troponin I.
MAb Isotypes:
IgG1 for MAbs 8E10, 16A11, 3C7, P4-14G5, M18, M155, M46, MF4, P4-9F6, 228, 625, 560, 596, 16A12, 415, 84, 581, 247, 17F3, p45-10, 909, 810, 820
IgG2a for MAbs 4C2,  267, 10F4 IgG2b for MAbs 19C7 and C5
IgG3 for MAbs 916, 801
IgM for MAb 458

Applications:
Cardiac troponin I immunoassay; cardiac troponin I immunoprecipitation, immunohistochemistry, immunoaffinity purification.
All MAbs work in Western blotting. For better sensitivity in Western blotting we recommend using MAbs 19C7, 16A11 and MF4.
Recommended pairs for quantitative sandwich immunoassay (capture – detection): 625 – 19C7, 560 – 458.
Best pairs: 19C7 – 16A11, 560 – 19C7
MAbs 801, 810, 820, 909 and 916 work well as capture MAbs with MAb 19C7 as detection MAb.
Antibody combinations for quantitative sandwich immunoassay:
19C7 + M18 (mixture for coating) – MF4 + 16A11 (mixture for detection)
M18 + 560 (mixture for coating) – MF4 + 19C7 (mixture for detection)
228 + 560 (mixture for coating) – 19C7+ 458 (mixture for detection)
M18 + 560 (mixture for coating) – 19C7 + 267 (mixture for detection)
MF4 + 560 (mixture for coating) – 19C7 + 267 (mixture for detection)
19C7 + MF4 (mixture for coating) – 7B9 (anti-TnC MAb; Cat. #2-TnC-h)

Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage Conditions:
+ 4 °C
Material Safety Note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
References:
Search Troponin references from PubMed.

See all Troponin I References

Click below to download a PDF product data sheet
   
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