Monoclonal mouse anti-neuron specific enolase (NSE)

Catalog Number: 2-NSE

From: $254.00

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General Category:

    Monoclonal antibodies » Neuroscience antibodies
    Monoclonal antibodies » Tumor Markers

Disease Category:

    Tumor Markers » Monoclonal antibodies
    Neuroscience » Monoclonal Antibodies
MAbs:
5G10, 5E2, 5A4, 1C1, H11, 2F1
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.
Specificity:
Human NSE
MAb Isotypes:
IgG2a for MAbs 5E2, 1C1, 5A4, H11 and 2F1
IgG2b for MAb 5G10
Applications:
MAbs 5G10, 5A4 and 5E2 react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts. MAbs may be used for immunohistochemistry on formalin-fixed and
paraffinembedded sections. Recommended dilution is 1:100 – 1:300. Optimal dilution must be determined by experimentation.
All MAbs work in ELISA. Recommended pairs for sandwich immunoassay (capture-detection):
5G10 – 1C1
5E2 – 1C1
1C1 – 5E2
5A4 – 1C1
H11 – 2F1
Purification:
Chromatography on protein G Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage Conditions:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
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