Monoclonal mouse anti-thyroxine

Catalog Number: 1-THR

From: $220.00

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General Category:

    Monoclonal antibodies » Hormones
    Monoclonal antibodies » Veterinary » Other animal specific antibodies

Disease Category:

    Hormones » Monoclonal antibodies
    Veterinary » Monoclonal antibodies » Other animal specific antibodies

1H1, XM212
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with conjugate of purified thyroxine (Sigma T-2501) with BSA.

Thyroxine (T4)
MAbs react with conjugate T4-BSA and with free thyroxine in ELISA. Cross-reactivity with triiodothyronine (Sigma T-2752) is less than 0.1%.
The reactivity of the MAb XM212 with L-thyroxine is 100 % and with D-thyroxine 30%.

MAb Isotypes:
IgG for MAb XM212
IgG1 for MAb 1H1
Detection of thyroxine. ELISA.
1H1 by chromatography on protein A Sepharose XM212 by ion exchange chromatography
1H1 in PBS, pH 7.4, 0.1 % sodium azide (NaN3) XM212 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % of sodium azide (NaN3)
Storage Conditions:
1H1 at + 4 °C
XM212 at + 4 °C (<10 months) or at – 20 °C (for longer period).

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.05 – 0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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