Catalog Number: 8-TIC-rh
Source:Expressed in E. coli.Protein sequence:Product of gene TNNI3; 210 a.a.r.
MADGSSDAAR EPRPAPAPIR RRSSNYRAYA TEPHAKKKSK ISASRKLQLK TLLLQIAKQE LEREAEERRGEKGRALSTRC QPLELAGLGF AELQDLCRQL HARVDKVDEE RYDIEAKVTK NITEIADLTQ KIFDLRGKFK RPTLRRVRIS ADAMMQALLG ARAKESLDLR AHLKQVKKED TEKENREVGD WRKNIDALSG MEGRKKKFES
Recombinant human cardiac TnI contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).Applications:Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnI standardAnalysis:Purity > 95 % (SDS-PAGE).
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) – 0.420 (measured at 280 nm).Immunological activity:Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnI isoform.Purification:Immunoaffinity purification followed by ion-exchange chromatographyPresentation:Lyophilized from 0.01 M HClReconstitution:Reconstitute with Tris/urea buffer (20 mM Tris pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol).Storage Conditions:Lyophilized -20 °C (between -15 and -30 °C allowed)
Reconstituted -70 °C (between -65 and -80 °C allowed)Other information:Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.Material Safety Note:This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
Antigens » Cardiac Markers » Troponin I and Troponin complex
Cardiac Markers » Antigens » Troponin I and Troponin complex
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