Search Results for: troponin I

[add_desc][title]Source:[/title][description]Human heart tissue.
Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.[/description][/add_desc] [add_desc][title]Applications:[/title][description]TnC is suitable for use as a standard, immunogen for antisera production, stabilizer of TnI in solutions, troponin biochemical and immunochemical studies, tracer for iodination.[/description][/add_desc] [add_desc][title]Analysis:[/title][description]Purity > 98 % (SDS-PAGE)[/description][/add_desc] [add_desc][title]Presentation:[/title][description]Lyophilized from protein solution in 150 mM KCl, 20mM Tris-HCl, pH 7.5.
Product is recommended to reconstitute with distilled water.[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]-20 °C (between -15 and -30 °C allowed)
-70 °C for long term storage (between -65 and -80 °C allowed)[/description][/add_desc] [add_desc][title]Material safety note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.[/description][/add_desc] [add_desc][title]Additional product information:[/title][description] Troponin I Assay Notes[/description][/add_desc] [add_desc][title]References:[/title][description] Search Troponin C references from PubMed.
See all Troponin references.[/description][/add_desc] [add_desc][title]Related Products:[/title][description]See other cardiac markers and blood coagulation products.[/description][/add_desc]

[add_desc][title]Synonym names:[/title][description]Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3[/description][/add_desc] [add_desc][title]Source:[/title][description]Expressed in E. coli[/description][/add_desc] [add_desc][title]Protein sequence:[/title][description]Product of gene TNNT2; 288 a.a.r.
MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN ММHFGGYIQK QAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK
Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).[/description][/add_desc] [add_desc][title]Applications:[/title][description]Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard[/description][/add_desc] [add_desc][title]Analysis:[/title][description]Purity > 95 % (SDS-PAGE).
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) – 0.490 (measured at 280 nm)[/description][/add_desc] [add_desc][title]Immunological activity:[/title][description]Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform[/description][/add_desc] [add_desc][title]Purification:[/title][description]Immunoaffinity purification followed by ion-exchange chromatography[/description][/add_desc] [add_desc][title]Presentation:[/title][description]Lyophilized from 0.01 M HCl[/description][/add_desc] [add_desc][title]Reconstitution:[/title][description]Reconstitute with Tris/urea buffer (20 mM Tris pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol.[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]Lyophilized -20 °C (between -15 and -30 °C allowed)
Reconstituted -70 °C (between -65 and -80 °C allowed)[/description][/add_desc] [add_desc][title]Other information:[/title][description]Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.[/description][/add_desc]

[add_desc][title]Source:[/title][description]Human skeletal muscle.
Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.[/description][/add_desc] [add_desc][title]Applications:[/title][description]Immunogen for antibody production, troponin biochemical and immunochemical studies.[/description][/add_desc] [add_desc][title]Analysis:[/title][description]Purity more than 95 % (SDS-PAGE).
Protein content determined by the Lowry procedure.
Immunological identity confirmed by reaction with monoclonal antibody that is specific for the TnT skeletal muscle isoform.[/description][/add_desc] [add_desc][title]Presentation:[/title][description]Lyophilized from protein solution in 0.01 M HCl.[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]-20 °C (between -15 and -30 °C allowed)
-70 °C for long term storage (between -65 and -80 °C allowed)[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.[/description][/add_desc] [add_desc][title]References:[/title][description] Search Troponin T references from PubMed.
See all Troponin references.[/description][/add_desc] [add_desc][title]Related Products:[/title][description]See other cardiac markers and blood coagulation products.[/description][/add_desc]

[add_desc][title]Immunogen:[/title][description]Human cardiac troponin T, purity more than 98%[/description][/add_desc] [add_desc][title]Host animal:[/title][description]Goat[/description][/add_desc] [add_desc][title]Specificity:[/title][description]Human cardiac troponin T[/description][/add_desc] [add_desc][title]Applications:[/title][description]Human cardiac troponin T immunoassay, immunoprecipitation, immunohistochemistry, Western Blotting[/description][/add_desc] [add_desc][title]Purification:[/title][description]Immunoaffinity method utilizing cTnT-agarose as an affinity matrix.[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.09 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C (between +2 and +8 °C allowed)[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc] [add_desc][title]References:[/title][description] Search Troponin T references from PubMed.

See other Troponin T references.

See other cardiac and  blood coagulation products.[/description][/add_desc]

Alternatives in vitro!
Please note that MAb 7B9 is also available as in vitro produced product under Cat.# 2-TnC-iv. For immunoassays utilizing this MAb, we recommend selecting the in vitro produced product.

[add_desc][title]MAbs:[/title][description]7B9, 12G3, 1A2
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cellsof Balb/c mice immunized with human cardiac troponin complex (MAbs 7B9, 12G3) or isolatedslow skeletal isoform of TnC (MAb 1A2).[/description]

[add_desc][title]Specificity:[/title][description]Human TnC. No cross-reactivity with cTnT and cTnI. All MAbs recognize TnC also in binary complex with cTnI.[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG1 for MAb 7B9
IgG2a for MAb 1A2
IgG2b for MAb 12G3[/description][/add_desc] [add_desc][title]Applications:[/title][description]TnC immunoassay and immunoaffinity purification.
All MAbs work in Western blotting.
Best pair for TnC sandwich immunoassay (capture-detection): 1A2 – 7B9
MAbs can be used in high sensitivity cTnI sandwich immunoassay in pairs with antibodies specificto human cTnI ( Cat.# 2-TIc) and Tn complex ( cat #2-TC).
Best pairs for Tn complex sandwich immunoassay (capture-detection): Tcom8 ( cat #2-TC) – 7B9
[/description][/add_desc] [add_desc][title]Purification:[/title][description]Chromatography on protein A Sepharose[/description][/add_desc] [add_desc][title]Presentation:[/title][description] PBS, pH 7.4, 0.1 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very smallappropriate care must be taken when handling this product.[/description][/add_desc] [add_desc][title]References:[/title][description]Search Troponin C references fromPubMed.[/description][/add_desc]

[add_desc][title]MAbs:[/title][description]7B9cc
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human cardiac troponin complex.[/description][/add_desc] [add_desc][title]Specificity:[/title][description]Human TnC. No cross-reactivity with cTnT and cTnI. MAb recognizes TnC also in binary complex with cTnI.[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG1 for MAb 7B9cc[/description][/add_desc] [add_desc][title]Purification:[/title][description]Chromatography on protein A Sepharose[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.1 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Other information:[/title][description]More antibodies against TnC under Cat.# 2-TnC-h.[/description][/add_desc] [add_desc][title]Material safety note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc]

[add_desc][title]MAbs:[/title][description]9G6, 7F4, 7G7, 2F3, 1A11, 1C11, 1F11, 7E7
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnT (MAb 2F3, 7G7, 1A11, 9G6, 1C11, 1F11, 7F4), bovine TnT or human Tn complex (MAb 7E7).[/description][/add_desc] [add_desc][title]Specificity:[/title][description]MAbs 2F3, 7F4, 7G7, 1A11, 9G6, 1C11 and 1F11 have no cross-reaction with skeletal TnT. Cross-reactivity of MAb 7E7 with cardiac TnI is 5 %.[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG1 for MAbs 9G6, 1C11, and 7G7
IgG2b for MAbs 2F3, 1A11, 7F4 and 1F11
IgG for MAb 7E7[/description][/add_desc] [add_desc][title]Applications:[/title][description]TnT immunoassay and TnT immunoaffinity purification. It is possible to use following pairs of antibodies for sandwich immunoassay (capture-detection): 1C11-9G6, 1C11-7F4, 7G7-9G6, 7G7- 1C11, 7G7-2F3, 1C11-7E7 Best capture antibodies for BIAcore are 1C11, 9G6, 7G7.[/description][/add_desc] [add_desc][title]Purification:[/title][description]Protein A Sepharose chromatography.[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.1 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc]

[add_desc][title]References:[/title][description]Search Troponin T references from PubMed.
See all Troponin T References. See references where Monoclonal mouse anti-cardiac Troponin T (cTnT) has been used in.[/description][/add_desc]

[add_desc][title]MAbs:[/title][description]20C6
New MAb: Tcom8
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human native troponin complex, isolated in mild conditions from human cardiac tissue.[/description][/add_desc] [add_desc][title]Specificity:[/title][description]Native cardiac troponin complex. No cross-reaction with individual components of troponin complex.
[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG1 for Tcom8
IgG2b for 20C6[/description][/add_desc]

[add_desc][title]Applications:[/title][description]Immunological and biochemical studies. Human cardiac Tn complex immunodetection in direct ELISA. MAbs can be used in high sensitivity cTnI sandwich immunoassay in pairs with antibodies specific to human cTnI (Cat.# 2-TIc) and TnC (Cat.# 2-TnC-h)

Best pairs for Tn complex immunodetection (capture-detection)Tcom8 – 7B9 Tcom8 – 7B9 (cat #2-TnC-h)19C7 (cat # 2-TIc)–20C6[/description][/add_desc]

[add_desc][title]Purification:[/title][description]Chromatography on protein A Sepharose[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.1 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc]

[add_desc][title]References:[/title][description]Search Troponin reference from PubMed.[/description][/add_desc]

New. Biomarkers to screen for mysterious heart damage and other health issues befalling some COVID-19 patients. Testing for SARS-CoV-2 is not the only requirement in regards to managing COVID-19. Screening and monitoring for the various health issues that may arise as part of COVID-19 disease management are also needed. You can find the following biomarkers in … Read more →

Cardiovascular diseases are a group of disorders that occur in the heart and blood vessels and are the world’s leading cause of death. Researchers are currently studying whether tests that detect levels of cardiac troponin I (cTnI) in the blood my help physicians diagnose heart attacks sooner. For over 20 years, Advanced ImmuunoChemical, Inc. has been at … Read more →

Troponin B-type natriuretic peptide IGFBP-4 and PAPP-A Transferrin receptor Adiponectin D-dimer Troponin Barth JH, et al. (2014) Recommendation to harmonize the units for reporting cardiac troponin results.Clin Chim Acta. 2014 May 15(432):166. Filatov VL, et al. (1998) Epitope mapping of anti-troponin I monoclonal antibodies. Biochem. Mol. Biol. Int. 45(6), 1179-1187. Filatov VL, et al. (1999) Troponin: structure, properties, … Read more →

Filatov VL, et al. (1998) Epitope mapping of anti-troponin I monoclonal antibodies. Biochem. Mol. Biol. Int. 45(6), 1179- 1187. Filatov VL, et al. (1999) Troponin: structure, properties, and mechanism of functioning. Biochemistry 64(9), 969-985. Katrukha A, et al. (1999) Biochemical factors in uencing measurement of cardiac troponin I in serum. Clin. Chem. Lab. Med. 37(11-12), … Read more →

Product Patents Patents related to diagnostics of cardiovascular diseases. Immunoassay Kit for Quantification of BNP and proBNP     US 9145459   The present invention relates to an immunoassay for detection of BNP, proBNP and fragments thereof. Essentially the assay comprises: a) contacting the antigen with a first antibody specific to a fragment corresponding to … Read more →

We offer monoclonal antibodies and recombinant antigens for diagnostic immunoassays for several animal diseases, including cardiovascular disease, inflammation, infectious and renal diseases. CANINE NEW! Canine C-reactive Protein (cCRP), Antibodies — (Cat #2-CRP-c) & Recombinant Canine C-reactive Protein (cCRP) — (Cat #7-CRP-c)      NEW! Canine Serum Amyloid A (SAA) — (Cat #2-SAA-a) & Recombinant Canine Serum Amyloid A … Read more →

 Advanced ImmunoChemical offers 5 monoclonal antibodies, in addition to the recombinant human and recombinant rabbit antigens, for the detection of glyceraldehyde-3-phosphate dehydrogenase, an enzyme known for its catalyzing role in glycolysis. These antibodies and antigens are sufficient for immunodetection of GAPDH by Western blotting, sandwich immunoassays, and other immunocytochemical processes. Click here to view GAPDH Product … Read more →

Citations: 2010 – 2016 [HTML] from nature.com Tissue kallikrein-modified human endothelial progenitor cell implantation improves cardiac function via enhanced activation of akt and increased angiogenesis Y Yao, Z Sheng, Y Li, C Fu, G Ma, N Liu, J Chao… – Laboratory Investigation, 2013 Abstract Endothelial progenitor cells (EPCs) have been shown to enhance angiogenesis not … Read more →

EZH2 inhibition blocks multiple myeloma cell growth through upregulation of epithelial tumor suppressor genes H Hernando, KA Gelato, R Lesche, G Beckmann… – Molecular cancer …, 2015 Abstract Multiple myeloma is a plasma cell malignancy characterized by marked heterogeneous genomic instability including frequent genetic alterations in epigenetic enzymes. In particular, the histone methyltransferase Enhancer of … Read more →

See references where our products have been used. Click the title of the article to read the whole article in a new browser window. Cat# 1-pINS: Monoclonal mouse anti-human proinsulin Back SH, et al. (2009) Translation Attenuation through eIF2 [alpha] Phosphorylation Prevents Oxidative Stress and Maintains the Differentiated State in [beta] Cells. Cell Metabolism 10(1), … Read more →

  Molecular Biology SmarTaq Polymerase AssayNotes Cardiac Markers Chimeric cardiac troponin I antibodies AssayNotes ProBNP and proBNP-derived peptides (BNP and NT-proBNP) AssayNotes Antibodies for New Type of BNP Immunoassay AssayNotes hsCRP AssayNotes PAPP-A AssayNotes Myoglobin AssayNotes Heart type fatty acid binding protein (H-FABP) AssayNotes Cystatin C AssayNotes Myeloperoxidase AssayNotes Lp-PLA2 AssayNote Cardiac Troponin (cTnT) AssayNotes … Read more →

Troponin Gomes, A.V, Potter,J.D., Szczesna-Cordary, D. (2002) The role of Troponin in muscle contraction. Life, 54: 323-333. Marston,S.B., Redwood,C.S. (2003) Modulation of thin fi lament activation by breakdown or isoform witching of thin fi lament Proteins. Circ. Res., 93: 1170-1178. Sarko J, Pollack CV Jr. (2002) Cardiac troponins. J Emerg Med. 2002, 23(1), pp. 57-65. … Read more →