Interleukin-6 (IL-6) has been shown to be involved in many physiologic activities, disease initiation and progression and the pleiotropic nature of this cytokine makes it a key player in many physiologic processes (1). Serum IL-6 levels have also upregulated during recent outbreaks of coronavirus and are associated with severity of COVID-19 infection (2). IL-6 could be a predictive marker of survival in COVID-19 patients outperforming CRP, D-dimer, and ferritin, independently of demographics and comorbidities (3).
Baseline levels of human IL-6 in the blood are known to be in single pg/ml digits and can increase up to thousands pg/ml upon severe sepsis, therefore assays characterized by high sensitivity and wide diagnostic window are needed for reliable determination of IL-6 in the bloodstream..
Advanced ImmunoChemical now offers 7 new MAb pairs to IL-6 that are suitable for high sensitivity IL-6 assay development (Cat#2-IL6 ) and capable of detecting both recombinant human IL-6 (Cat. #8-hIL6) and native IL-6 in serum. Monoclonal antibodies were developed using full length human recombinant IL-6 as an immunogen and mice, rats, and rabbits as the source of the immune cells. All of the developed MAbs are capable of working in a sandwich chemiluminescent assay with streptavidin -polyHRP and have low cross-reactivity to other cognate interleukin human proteins, which secures a very high assay specificity as shown below:
Figure 1. Linearity of dilution using new IL-6 MAb
Dilution linearity of IL-6 from septic patient’s plasma was measured in sandwich CLIA with MAb pair L152-L137. Plasma IL-6 concentration was measured with Roche Cobas 6000 analyser. The LoD of the MAb pair L152-L137 is 0.5 pg/mL
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1. Del Giudice M and Gangestad SW: Rethinking IL 6 and CRP: Why they are more than inflammatory biomarkers, and why it matters. 2018, Brain Behav Immun 70: 61 75.
2. Gong J, Dong H, Xia SQ, et al. Correlation analysis between disease severity and inflammation-related parameters in patients with COVID-19 pneumonia [published online February 27, 2020]. medRxiv. doi:10.1101/2020.02 .25.20025643
3. Diane Marie Del Valle et al, An inflammatory cytokine signature predicts COVID-19 severity and survival. 2020, Nature Medicine 26, 1636–1643.
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