Search Results for: BNP

[add_desc][title]MAbs:[/title][description]15F11cc, 13G12cc, 15C4cc, 24E11cc, 29D12cc, 18H5cc, NT34cc, 5B6cc, 7B5cc, 11D1cc, 16E6cc

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (MAbs 5B6cc,  29D12cc), a.a.r. 13-27 (MAbs 15F11cc, 13G12cc, 18H5cc, 7B5cc), a.a.r. 22-36 (MAb NT34cc) a.a.r. 28-45 (11D1cc, 16E6cc) and a.a.r. 61-76 (MAbs 24E11cc, 15C4cc). The peptides used for immunization were conjugated with carrier protein.[/description]

[add_desc][title]Specificity:[/title][description]Human NT-proBNP and proBNP.[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]
IgG1 for MAb 18H5cc, NT34cc, 5B6cc, 7B5cc, 11D1cc, 16E6cc

IgG2a for MAbs 29D12cc, 13G12cc, 24E11cc

IgG2b for MAbs 15F11cc, 15C4cc[/description][/add_desc] [add_desc][title]Applications:[/title][description]NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.
Best pairs for sandwich immunoassay (capture-detection): 15F11cc – 24E11cc, 15C4cc – 29D12cc, 15C4cc – 13G12cc, 15C4cc – 18H5cc, 29D12cc-NT34cc. These pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.

MAbs specific to peptides 5-12 (MAb 29D12cc), 13-24 (MAb 15F11cc), 15-20 (MAbs 13G12cc, 18H5cc) and 25-34 (MAb NT34cc) and 15-21 (7B5cc) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2). The best pair (capture-detection):

50E1cc (Cat.# 4BNP2) – 18H5cc (Cat.# 4NT1cc))[/description][/add_desc] [add_desc][title]Purification:[/title][description]Chromatography on protein A Sepharose[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.09 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+4 °C (+2 … +8 °C allowed)[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc]

Heart failure is a severe health problem that is characterized by an increasing prevalence and economic cost. According to the American Heart Association, in the United States nearly one million new cases are reported each year. BNP and NT-proBNP are established diagnostic and prognostic biomarkers of heart failure. The clinical values of these markers are similar and … Read more →

New. Anti-BNP Antibodies Advanced ImmunoChemical announces three new monoclonal antibodies specific to BNP. BNP is an established cardiac biomarker and used as a diagnostic and prognostic marker of heart failure. We now provide eight MAbs specific to BNP as shown in Figure 1. Figure 1. New MAbs, 100cc, 130cc and 429cc for use in combination … Read more →

Brain natriuretic peptide (BNP) and the N-terminal part of the BNP precursor proBNP (NT-proBNP) are established diagnostic and prognostic biomarkers of heart failure (HF). HF is a severe clinical and public health problem that is characterized by an increasing prevalence and economic cost. Chronic heart disease, hypertension and diabetes are among the known risk factors … Read more →

Advanced ImmunoChemical offers antigens and corresponding high affinity monoclonal antibodies specific to recombinant human BNP, proBNP, and NT-proBNP for the development of sensitive, reliable immunoassays specific to regions not affected by glycosylation during proBNP processing. Also a New Novel Single Epitope Sandwich (SES) assay for precise quantification of circulating BNP and proBNP in HF patients. … Read more →

Advanced ImmunoChemical offers 11 monoclonal antibodies and the recombinant antigen for the detection of N-terminal pro-B-type natriuretic peptide (NT-proBNP) in canines. These products are sufficient for immunoassay development for the determination of NT-proBNP levels in canine blood. All antibodies offered have shown to identify both the recombinant and native NT-proBNP in canine blood. Click here to … Read more →

[add_desc][title]Source:[/title][description]Recombinant protein with N-terminal proprietary affinity tag expressed n E.coli.[/description][/add_desc] [add_desc][title]Applications:[/title][description]Standard for the calibration of canine NT-proBNP immunoassatys.[/description][/add_desc] [add_desc][title]Analysis:[/title][description]

Recombinant NT-proBNP corresponds to the fragment 1-85 a.a.r. of canine proBNP and contains additional affinity tag sequence of 16 a.a.r. at the N-terminus.

 

Totally, recombinant NT-proBNP conists of 101 a.a.r.

 

Predicted molecular mass 10545 Da is confirmed by MALDI-MS.

 

Purity > 95% (Tricine-SDS-PAGE).

 

Concentration is determined spectrophotometrically.

 

Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of canine NT-proBNP molecule (Cat# 2-CNT).

 

[/description][/add_desc] [add_desc][title]Presentation:[/title][description]

10 mM potassium phosphate, pH 7.4, 150 mM NaCl.

[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]

-70 °C (-65 … -80 °C allowed)

[/description][/add_desc] [add_desc][title]Other information:[/title][description]

Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.

To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tric-HCl, pH 7.8, 0.15 M NaCl, 0.01% Tween 40, 0.5% bovine serum albumin).

[/description][/add_desc] [add_desc][title]Material safety note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.[/description][/add_desc] [add_desc][title]Additional Product Information:[/title][description]

Canine NT-proBNP Assay Notes

[/description][/add_desc]

 

[add_desc][title]References:[/title][description]

Search NT-proBNP canine references from PubMed

[/description][/add_desc] [add_desc][title]Related Products:[/title][description]

Cat# 2-CNT Monoclonal mouse anti-canine N-terminal proBNP (NT-proBNP)

[/description][/add_desc]

 

[add_desc][title]MAbs:[/title][description]Ab-BNP2cc
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with immune complex formed by Fab-fragment of BNP-specific MAb 24C5 (Cat.# 2-BNP) and synthetic human BNP.[/description][/add_desc] [add_desc][title]Specificity:[/title][description]Human BNP and proBNP in immune complex with MAb 24C5[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG2a for MAb Ab-BNP2cc[/description][/add_desc] [add_desc][title]Applications:[/title][description]BNP and proBNP sandwich immunoassays (detection), only as a pair to MAb 24C5.[/description][/add_desc] [add_desc][title]Purification:[/title][description]Protein A affinity chromatography[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.09 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Other information:[/title][description]More antibodies against immune complex (24C5-BNP/proBNP) under Cat.# 2-ICBNP.[/description][/add_desc] [add_desc][title]Material safety note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.09 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc]

[add_desc][title]Source:[/title][description]Recombinant E.coli.[/description][/add_desc] [add_desc][title]Applications:[/title][description]Calibrator or standard in immunoassays, immunogen for antibody production.[/description][/add_desc] [add_desc][title]Analysis:[/title][description]Purity > 95 % (Tricine-SDS-PAGE).
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of proBNP molecule.
Predicted molecular mass 12036 Da confirmed by MALDI-MS.
Recombinant human proBNP contains additional methionine residue at the N-terminus (in comparison with native proBNP).
Human recombinant proBNP also contains one intramolecular disulfide bond between residues Cys87 and Cys103.[/description][/add_desc] [add_desc][title]Purification:[/title][description]Immunoaffinity purification followed by ion-exchange chromatography[/description][/add_desc] [add_desc][title]Presentation:[/title][description]10 mM potassium phosphate, pH 7.4, 150 mM NaCl.[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]-70 °C (between -65 and -80 °C allowed)[/description][/add_desc] [add_desc][title]Other information:[/title][description]Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
To prepare diluted samples of proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).[/description][/add_desc] [add_desc][title]Material safety note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.[/description][/add_desc]

[add_desc][title]Additional product information:[/title][description]Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) Assay Notes.
Antibodies for New Type of BNP immunoassay – Single Epitope Sandwich Assay Notes.[/description][/add_desc][add_desc][title]References:[/title][description] Search proBNP recombinant references from PubMud.
See all proBNP References.[/description][/add_desc]

[add_desc][title]Related Products:[/title][description]See other cardiac markers and blood coagulation products.[/description][/add_desc]

[add_desc][title]Source:[/title][description]Recombinant E.coli.[/description][/add_desc] [add_desc][title]Applications:[/title][description]Calibrator or standard in immunoassays, immunogen for antibody production.[/description][/add_desc] [add_desc][title]Analysis:[/title][description]Purity > 95 % (Tricine-SDS-PAGE).
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.
Predicted molecular mass 8589 Da confirmed by MALDI-MS.
Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).[/description][/add_desc] [add_desc][title]Purification:[/title][description]Immunoaffinity purification followed by ion-exchange chromatography.[/description][/add_desc] [add_desc][title]Presentation:[/title][description]10 mM potassium phosphate, pH 7.4, 150 mM NaCl[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]-70 °C (between -65 and -80 °C allowed)[/description][/add_desc] [add_desc][title]Other information:[/title][description]Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).[/description][/add_desc] [add_desc][title]Material safety note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.[/description][/add_desc]

[add_desc][title]Additional product information:[/title][description] Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) Assay Notes.
Antibodies for New Type of BNP immunoassay – Single Epitope Sandwich Assay Notes.[/description][/add_desc][add_desc][title]References:[/title][description] Search NT-proBNP recombinant references from PubMud.
See all NT-proBNP Reference[/description][/add_desc]

[add_desc][title]Related Products:[/title][description]See other cardiac markers and blood coagulation products.[/description][/add_desc]

[add_desc][title]Applications:[/title][description]Calibrator or standard in immunoassays, immunogen for antibody production[/description][/add_desc] [add_desc][title]Analysis:[/title][description]Purity > 95% (Tricine-SDS-PAGE in reducing conditions).
ProBNP concentration is determined spectrophotometrically[/description][/add_desc] [add_desc][title]Purification:[/title][description]Immunoaffinity purification followed by ion-exchange[/description][/add_desc] [add_desc][title]Presentation:[/title][description]Lyophilized from 300 mM NaCl, 50 mM sodium acetate, pH 4.2.[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]Before and after reconstitution at -70 °C (between -65 and -80 °C allowed)[/description][/add_desc] [add_desc][title]Other information:[/title][description]Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.
To avoid loss of immunological activity it is recommended to reconstitute lyophilized protein in 50 mM sodium acetate, pH 4.2 to obtain the same proBNP concentration that was before lyophilization. After reconstitution store at -70 °C.[/description][/add_desc]

[add_desc][title]Material safety note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.[/description][/add_desc][add_desc][title]References:[/title][description]  Search Human recombinant proBNP glycosylated references from PubMud.
See all proBNP, BNP and NT-ProBNP References.[/description][/add_desc]

[add_desc][title]Source:[/title][description]Human cardiPooled normal human EDTA plasma.
Blood samples from donors were tested and found to be negative for HBsAg, HIV-1 and HIV-2 antibodies and HCV.[/description][/add_desc] [add_desc][title]Applications:[/title][description]BNP and NT-proBNP free plasma is free of whole proBNP molecule and any proBNP fragments (BNP and NT-proBNP). It can be used for preparation of any proBNP-related standards and calibrators for immunoassays based on detection of any kinds of proBNP-related peptides.[/description][/add_desc] [add_desc][title]Purification:[/title][description]Immunoaffinity chromatography of pooled normal human plasma.
Gel for affinity column was prepared by coupling anti-BNP and anti-NT-proBNP monoclonal antibodies with different epitope specificity with BrCN-activated Sepharose CL 4B.[/description][/add_desc] [add_desc][title]Presentation:[/title][description]Frozen liquid.[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]-20 °C (between -15 and -30 °C allowed)[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.[/description][/add_desc] [add_desc][title]References:[/title][description] Search for BNP and NT-proBNP references from PubMed.[/description][/add_desc] [add_desc][title]Related products:[/title][description]Cat.# 2-BNP: Brain natriuretic peptide (BNP) MoAb.
See other cardiac markers and blood coagulation products.[/description][/add_desc]

[add_desc][title]MAbs:[/title][description]CaNT89, CaNT90*, CaNT19, CaNT46, CaNT49, CaNT53*
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with:
 recombinant canine NT-proBNP with tag expressed in E. coli (CaNT90)
 recombinant canine NT-proBNP expressed in E. coli as a fusion protein with carrier (CaNT89, CaNT19, CaNT46, CaNT925)
 synthetic peptides corresponding to 3-26 a.a.r., 13-33 a.a.r., and 64- 80 a.a.r. (CaNT49, CaNT53) of canine NT-proBNP[/description][/add_desc] [add_desc][title]Specificity:[/title][description]Canine NT-proBNP and proBNP.[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG1 for MAbs CaNT89, CaNT90, CaNT19, CaNT46, CaNT49, CaNT53
[/description][/add_desc] [add_desc][title]Applications:[/title][description]Quantification of canine NT-proBNP in plasma of dogs. Recommended pairs for sandwich immunoassay (capture-detection):
CaNT90 – CaNT89
CaNT19 – CaNT89
CaNT90 – CaNT53.
Extended list of two-site antibody combinations for detection of canine NT-proBNP in plasma can be provided as a supplementary material.[/description][/add_desc] [add_desc][title]Purification:[/title][description]Chromatography on protein A Sepharose[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.1 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
* Some applications in which these products may be used are covered by patents issued and applicable in certain countries. Because purchase of these products does not include a license to perform any patented application, users of these products may be required to obtain a patent license, depending on the particular application and country in which the product is used.[/description][/add_desc] [add_desc][title]Additional product information:[/title][description] Canine NT-proBNP Assay Notes[/description][/add_desc]

[add_desc][title]MAbs:[/title][description]15D7, 28F8, 16F3
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 13-27 (MAbs 16F3), a.a.r. 28-45  (MAb 15D7) and a.a.r. 61-76 (MAbs 28F8). The peptides used for immunization were conjugated with carrier protein.[/description]

[add_desc][title]Specificity:[/title][description]Human NT-proBNP and proBNP.[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG1 for MAbs 15D7, 16F3
IgG2a for MAb 28F8
[/description][/add_desc] [add_desc][title]Applications:[/title][description]NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.
These pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.
MAbs specific to peptides 13-27 (MAb 16F3) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 2-BNP). The best pairs (capture-detection):
50E1 (Cat.# 2-BNP) – 16F3 (Cat.# 2-NT-proBNP)
[/description][/add_desc] [add_desc][title]Purification:[/title][description]Chromatography on protein A Sepharose[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.1 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc] [add_desc][title]Additional product information:[/title][description] Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) Assay Notes. Antibodies for New Type of BNP immunoassay Single Epitope Sandwich Assay Notes.[/description][/add_desc] [add_desc][title]References:[/title][description]
Search NT-proBNP references from  PubMed. See all NT-proBNP References.[/description][/add_desc]

[add_desc][title]MAbs:[/title][description]24C5cc
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells ofBalb/c mice immunized with synthetic BNP peptide 11FGRKMDRISSSS22 conjugated with carrier protein.[/description]

[add_desc][title]Specificity:[/title][description]Human BNP and proBNP.[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG1 for MAbs 24C5cc[/description][/add_desc] [add_desc][title]Applications:[/title][description]BNP and proBNP immunoassay.[/description][/add_desc] [add_desc][title]Purification:[/title][description]Chromatography on protein A Sepharose[/description][/add_desc] [add_desc][title]Presentation:[/title][description] PBS, pH 7.4, 0.09 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Other information:[/title][description]More antibodies against BNP under Cat.# 2-BNP.[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.09 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc]

Alternatives in vitro!
Please note that MAb 24C5 is also available as in vitro produced product under Cat.# 2-BNP-iv. For immunoassays utilizing this MAb, we recommend selecting the in vitro produced product.

[add_desc][title]MAbs:[/title][description]26E2
Hybridoma clones has been derived from hybridization of Sp2/0 myeloma cells with spleen cellsof Balb/c mice immunized with human synthetic BNP peptiden 11FGRKMDRISSSS22  conjugated with carrier protein.[/description]

[add_desc][title]Specificity:[/title][description]Human BNP and proBNP.[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG1 for MAb 26E2
[/description][/add_desc] [add_desc][title]Applications:[/title][description]BNP and proBNP immunoassay. MAb recognizes BNP and proBNP in sandwich immunoassay.
MAb 26E2 react with proBNP and BNP in Western blotting.
Recommended pairs for BNP sandwich immunoassay (capture-detection): 50E1cc – 26E2 Sensitivity of the pair recognizes with high sensitivity the antigen in plasma samples of patients with heart failure.
[/description][/add_desc] [add_desc][title]Purification:[/title][description]Chromatography on protein A Sepharose[/description][/add_desc] [add_desc][title]Presentation:[/title][description]Presentation: PBS, pH 7.4, 0.09 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Material Safety Note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.09 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc] [add_desc][title]Additional product information:[/title][description] Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) Assay Notes. Antibodies for New Type of BNP immunoassay Single Epitope Sandwich Assay Notes.[/description][/add_desc] [add_desc][title]References:[/title][description]Search BNP references from  PubMed.[/description][/add_desc]

Alternatives in vitro!
Please note that MAb 24C5 is also available as in vitro produced product under Cat.#2-BNP-iv..Please note that MAb 24C5 is also available as in vitro produced product under Cat.# 2-BNP-iv. For immunoassays utilizing this MAb, we recommend selecting the in vitro produced product.

[add_desc][title]MAbs:[/title][description]Ab-BNP2, Ab-BNP4
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with immune complex formed by Fab-fragment of BNP-specific MAb 24C5 (Cat.# 2-BNP) and synthetic human BNP.[/description][/add_desc] [add_desc][title]Specificity:[/title][description]Human BNP and proBNP in immune complex with MAb 24C5[/description][/add_desc] [add_desc][title]MAb Isotypes:[/title][description]IgG2a for MAbs Ab-BNP2 and Ab-BNP4 for MAbs Ab-BNP2 and Ab-BNP4[/description][/add_desc] [add_desc][title]Applications:[/title][description]BNP and proBNP sandwich immunoassays (detection), only as a pair to MAb 24C5.[/description][/add_desc] [add_desc][title]Purification:[/title][description]Protein A affinity chromatography[/description][/add_desc] [add_desc][title]Presentation:[/title][description]PBS, pH 7.4, 0.1 % sodium azide (NaN3)[/description][/add_desc] [add_desc][title]Storage Conditions:[/title][description]+ 4 °C[/description][/add_desc] [add_desc][title]Material safety note:[/title][description]This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.[/description][/add_desc] [add_desc][title]Additional product information:[/title][description] Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) Assay Notes. Antibodies for New Type of BNP immunoassay Single Epitope Sandwich Assay Notes.[/description][/add_desc] [add_desc][title]References:[/title][description]Search Immune complex 24C5-BNP proBNP references from PubMed.[/description][/add_desc]

Click here to download the Canine NT-proBNP Assay Note (PDF). NT-proBNP measurement has been introduced into vet¬erinary practice during the last decade. NT-proBNP lev¬els are elevated in dogs with mitral valve disease and di¬lated cardiomyopathy. High¬est concentrations are ob¬served in dogs developing congestive heart failure. NT-proBNP measurement helps to discriminate congestive heart failure from primary … Read more →

Brain natriuretic peptide (BNP) is an acknowledged marker of heart failure (HF) that is widely used in clinical practice for HF diagnosis and patient management. BNP is known as an unstable molecule (1, 2). Several recent studies have revealed that BNP is presented by multiple forms in HF patients’ plasma, truncated from both N- and … Read more →

Click here to download the ProBNP and proBNP-derived peptides Assay Note (PDF). PreproBNP is composed of 134 amino acid residues (a.a.r.) and is synthesized in cardiac myocytes. Removal of signal peptide results in the appearance of proBNP molecule. Then proBNP is cleaved by unknown protease forming two peptides; BNP and N-terminal part of the proBNP. … Read more →